General Cell Lines and Cell Cultures
Cell Applications Inc Proliferating Rat Osteoblasts (ROb), Adult
Proliferating Rat Osteoblasts (ROb), Adult
Cell Applications Inc Rat Astrocytes Total Kit, neonatal
Rat Astrocytes Total Kit, neonatal:(R882A-05n) Cryopreserved (RA), neonatal, 1 vial(R821-500) Rat Astrocyte Growth Media, 500ml(090K) Subculture Reagent Kit
Eurofins Discoverx ChemiScreen Rhesus Macaque CCR5 Chemokine Receptor Membrane Preparation, 1 vial. (HTS010M)
The ChemiScreen Rhesus Macaque CCR5 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCR5 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay MRGPRD Receptor Frozen Cells, 2 Vials of Cells. (HTS206RTA)
The Ready-to-Assay MRGPRD Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. β-alanine) based activation of MRGPRD GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MRGPRD alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MRGPRD, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen M1 Acetylcholine Muscarinic Receptor Stable Cell Line, 2 vials. (HTS044C)
The ChemiScreen M1 Acetylcholine Muscarinic Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
STEMCELL Technologies G-CSF and Plerixafor Mobilized Human Peripheral Blood Mononuclear Cells, Frozen, Size: 5 x 10^6 cells
Start your assays with ready-to-use, ethically sourced, mobilized primary mononuclear cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications. Donors are mobilized with G-CSF, plerixafor, or G-CSF and plerixafor prior to collection. Isolated from leukapheresis samples using density gradient separation or red blood cell lysis and cryopreserved in CryoStorCS10 medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. ACDA is added as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.
Quidel Corporation Normal human serum depleted of complement C5 protein.
Normal human serum depleted of complement C5 protein.
ACROBiosystems HEK293/Human EpCAM Stable Cell Line
EpCAM is also known as CO171A, EGP, EGP40,GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy.Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
Cayman Chemical Glucagon RECPTR ntagonIst
A competitive antagonist of GCGR (IC50 = 181 nM); blocks glucagon-induced glycogenolysis in primary human hepatocytes, isolated liver, and humanized mice
ATCC ES-D3 (D3); Stem Cell; Mouse (Mus muscullus)
ES-D3 [D3] is an embryonic multipotent stem cell line that was isolated from the embryo of a mouse. This cell line was deposited by T Doetschman and can be used in stem cell research.
ILEX LIFE SCIENCES LLC EMEM FIBROBLASTS 6 X 500 ML
PAN-Biotech EMEM Cell Culture Medium for Fibroblasts, w/o: L-Glutamine, w: 2.2 g/L NaHCO3 (6 x 500 ml). BICARBONATE: With 2.2 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: With Phenol Red. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: EMEM Fibroblasts is a cell culture medium based on MEM Eagle (Eagle's Minimum Essential Medium) which was in turn developed from BME (Basal Medium Eagle). EMEM Fibroblasts is optimized specifically for the cultivation of fibroblasts. EMEM Fibroblasts is based on EMEM supplemented with additional or higher amount of amino acids and vitamins and is thus optimized for improved growth of fibroblasts. For cultivation of fibroblasts, this medium must be supplemented with 10% FBS before use.
ATCC Non-Enzymatic Cell Dissociation Media, 1X, 100 ML
Trypsin is the most commonly used enzyme for harvesting cells in culture. A non-enzymatic approach is needed when non-protein and animal-component free materials are required. ATCC Non-Enzymatic Cell Dissociation Solution is a sterile, phenol-red free solution composed of a proprietary mixture of chelators representing an optimized alternative to protein-digesting enzymes. This product is totally free of animal-derived components. This product has applications for cell culture, cell growth, and viability.
Eurofins Discoverx cAMP Hunter CHO-K1 HTR1A Gi Cell Line, 2 vials. (95-0132C2)
The cAMP Hunter HTR1A Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR1A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HTR1A, a naturally Gi/Go coupled GPCR. Upon activation of HTR1A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mGLP1R β-Arrestin Cell Line, 2 vials. (93-0478C2)
The PathHunter mGLP1R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGLP1R and EA-tagged β-Arrestin-2. Activation of mGLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC SK-N-DZ; Neuroblastoma; Human
SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. It has applications in immunology and neuroscience.